Au JLS, Kumar RR, Li D and Wientjes MG Kinetics of Hallmark Biochemical Changes in Paclitaxel-induced Apoptosis AAPS PharmSci 1999;
1
(3)
article 8
(https://www.pharmsci.org/scientificjournals/pharmsci/journal/99_8.html).
Figures and Tables
Figure 1.Time course of cell detachment. Panel A shows changes in total number (attached plus detached) of PC3 cells with time. Cells received continuous treatment with 0 nM (control, circles) and 20 nM paclitaxel (squares). Panel B shows kinetics of cell detachment. The numbers of cells that remained attached (open symbols) to the culture flask and the numbers of cells that were detached (solid symbols), with or without continuous treatment with 20 nM paclitaxel, were determined, and expressed as percentage of the total cell number. The average initial cell number was between 1 and 1.5 × 106. At the end of the 96-hour experiment, the average cell number was 1.5 × 107 for the untreated controls and between 1 and 1.5 & 106 for the paclitaxel-treated cells. Mean ± SD (n = 5).
Figure 2.Activation of caspase-3-like proteases. The activity of caspase-3-like proteases was determined using the ApoAlert CPP32 assay. The caspase activity remained constant over time in the untreated controls. Caspase activity induced by continuous treatment with 20 nM paclitaxel is expressed as % of the control values. Mean ± SD (n = 6). Detached cells, solid symbols; attached cells; open symbols.
Figure 3.PARP cleavage. Cells were treated with 20 nM paclitaxel continuously. Cleavage of PARP (116,000 d) to its proteolytic product (85,000 d), as a function of time, was monitored using Western blotting. Molecular markers of 112,000 d and 85,000 d are noted on the right.
Figure 4.Cytoplasmic DNA-histone complex. Cells were treated with 20 nM paclitaxel continuously. Release of DNA-histone complex to the cytoplasm, as a function of time, was monitored by the optical density at 405 nm using ELISA (n = 3).
Figure 5.Cells were treated with 20 nM paclitaxel continuously. DNA fragmentation, as a function of time, was monitored using gel electrophoresis
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